| City Name | Population | State | Rank |
|---|
| Memphis City | 646,889 | Tennessee | 1 |
| Nashville-davidson Metropolitan Government (balance) | 601,222 | Tennessee | 2 |
| Knoxville City | 178,874 | Tennessee | 3 |
| Chattanooga City | 167,674 | Tennessee | 4 |
| Clarksville City | 132,929 | Tennessee | 5 |
| Murfreesboro City | 108,755 | Tennessee | 6 |
| Jackson City | 65,211 | Tennessee | 7 |
| Johnson City | 63,152 | Tennessee | 8 |
| Franklin City | 62,487 | Tennessee | 9 |
| Bartlett City | 54,613 | Tennessee | 10 |
| Hendersonville City | 51,372 | Tennessee | 11 |
| Kingsport City | 48,205 | Tennessee | 12 |
| Collierville Town | 43,965 | Tennessee | 13 |
| Cleveland City | 41,285 | Tennessee | 14 |
| Smyrna Town | 39,974 | Tennessee | 15 |
| Germantown City | 38,844 | Tennessee | 16 |
| Brentwood City | 37,060 | Tennessee | 17 |
| Columbia City | 34,681 | Tennessee | 18 |
| La Vergne City | 32,588 | Tennessee | 19 |
| Cookeville City | 30,435 | Tennessee | 20 |
| Gallatin City | 30,278 | Tennessee | 21 |
| Oak Ridge City | 29,330 | Tennessee | 22 |
| Morristown City | 29,137 | Tennessee | 23 |
| Spring Hill City | 29,036 | Tennessee | 24 |
| Maryville City | 27,465 | Tennessee | 25 |
| Bristol City | 26,702 | Tennessee | 26 |
| Lebanon City | 26,190 | Tennessee | 27 |
| Mount Juliet City | 23,671 | Tennessee | 28 |
| East Ridge City | 20,979 | Tennessee | 29 |
| Farragut Town | 20,676 | Tennessee | 30 |
| Shelbyville City | 20,335 | Tennessee | 31 |
| Tullahoma City | 18,655 | Tennessee | 32 |
| Dyersburg City | 17,145 | Tennessee | 33 |
| Springfield City | 16,440 | Tennessee | 34 |
| Goodlettsville City | 15,921 | Tennessee | 35 |
| Greeneville Town | 15,062 | Tennessee | 36 |
| Sevierville City | 14,807 | Tennessee | 37 |
| Dickson City | 14,538 | Tennessee | 38 |
| Elizabethton City | 14,176 | Tennessee | 39 |
| Mcminnville City | 13,605 | Tennessee | 40 |
| Athens City | 13,458 | Tennessee | 41 |
| Soddy-daisy City | 12,714 | Tennessee | 42 |
| Middle Valley | 12,684 | Tennessee | 43 |
| Lakeland City | 12,430 | Tennessee | 44 |
| Red Bank City | 11,651 | Tennessee | 45 |
| Arlington Town | 11,517 | Tennessee | 46 |
| Portland City | 11,480 | Tennessee | 47 |
| Martin City | 11,473 | Tennessee | 48 |
| Lewisburg City | 11,100 | Tennessee | 49 |
| Seymour | 10,919 | Tennessee | 50 |
| Union City | 10,895 | Tennessee | 51 |
| Crossville City | 10,795 | Tennessee | 52 |
| Lawrenceburg City | 10,428 | Tennessee | 53 |
| Brownsville City | 10,292 | Tennessee | 54 |
| White House City | 10,255 | Tennessee | 55 |
| Millington City | 10,176 | Tennessee | 56 |
| Paris City | 10,156 | Tennessee | 57 |
| Manchester City | 10,102 | Tennessee | 58 |
| Bloomingdale | 9,888 | Tennessee | 59 |
| Clinton City | 9,841 | Tennessee | 60 |
| Covington City | 9,038 | Tennessee | 61 |
| Lenoir City | 8,642 | Tennessee | 62 |
| Winchester City | 8,530 | Tennessee | 63 |
| Humboldt City | 8,452 | Tennessee | 64 |
| Alcoa City | 8,449 | Tennessee | 65 |
| Ripley City | 8,445 | Tennessee | 66 |
| Atoka Town | 8,387 | Tennessee | 67 |
| Collegedale City | 8,282 | Tennessee | 68 |
| Jefferson City | 8,047 | Tennessee | 69 |
| Hartsville/trousdale County | 7,870 | Tennessee | 70 |
| Pulaski City | 7,870 | Tennessee | 71 |
| Milan City | 7,851 | Tennessee | 72 |
| Harrison | 7,769 | Tennessee | 73 |
| Fairview City | 7,720 | Tennessee | 74 |
| Lexington City | 7,652 | Tennessee | 75 |
| Signal Mountain Town | 7,554 | Tennessee | 76 |
| La Follette City | 7,456 | Tennessee | 77 |
| Dayton City | 7,191 | Tennessee | 78 |
| Fairfield Glade | 6,989 | Tennessee | 79 |
| Savannah City | 6,982 | Tennessee | 80 |
| Newport City | 6,945 | Tennessee | 81 |
| Colonial Heights | 6,934 | Tennessee | 82 |
| South Cleveland | 6,912 | Tennessee | 83 |
| Fayetteville City | 6,827 | Tennessee | 84 |
| Church Hill City | 6,737 | Tennessee | 85 |
| Oakland Town | 6,623 | Tennessee | 86 |
| Green Hill | 6,618 | Tennessee | 87 |
| Millersville City | 6,440 | Tennessee | 88 |
| Greenbrier Town | 6,433 | Tennessee | 89 |
| Lynchburg, Moore County Metropolitan Government | 6,362 | Tennessee | 90 |
| Harriman City | 6,350 | Tennessee | 91 |
| Henderson City | 6,309 | Tennessee | 92 |
| Erwin Town | 6,097 | Tennessee | 93 |
| Kingston City | 5,934 | Tennessee | 94 |
| Munford City | 5,927 | Tennessee | 95 |
| Pigeon Forge City | 5,875 | Tennessee | 96 |
| Nolensville Town | 5,861 | Tennessee | 97 |
| Tellico Village | 5,791 | Tennessee | 98 |
| Sweetwater City | 5,764 | Tennessee | 99 |
| Rockwood City | 5,562 | Tennessee | 100 |
| Mount Carmel Town | 5,429 | Tennessee | 101 |
| Bolivar City | 5,417 | Tennessee | 102 |
| Loudon Town | 5,381 | Tennessee | 103 |
| Mckenzie City | 5,310 | Tennessee | 104 |
| Eagleton Village | 5,052 | Tennessee | 105 |
| Jonesborough Town | 5,051 | Tennessee | 106 |