| City Name | Population | State | Rank |
|---|---|---|---|
| Bristol City | 996 | Florida | 1 |
| Center Hill City | 988 | Florida | 2 |
| Rio | 965 | Florida | 3 |
| Homestead Base | 964 | Florida | 4 |
| Cottondale Town | 933 | Florida | 5 |
| Crescent Beach | 931 | Florida | 6 |
| Lake Kathryn | 920 | Florida | 7 |
| Golden Beach Town | 919 | Florida | 8 |
| Pomona Park Town | 912 | Florida | 9 |
| Palm Shores Town | 900 | Florida | 10 |
| Allentown | 894 | Florida | 11 |
| Grand Ridge Town | 892 | Florida | 12 |
| Jennings Town | 878 | Florida | 13 |
| Pine Lakes | 862 | Florida | 14 |
| Sorrento | 861 | Florida | 15 |
| Franklin Park | 860 | Florida | 16 |
| Stacey Street | 858 | Florida | 17 |
| Chumuckla | 850 | Florida | 18 |
| Greenville Town | 843 | Florida | 19 |
| Medley Town | 838 | Florida | 20 |
| Harold | 823 | Florida | 21 |
| Paisley | 818 | Florida | 22 |
| Jupiter Island Town | 817 | Florida | 23 |
| Panacea | 816 | Florida | 24 |
| Andrews | 798 | Florida | 25 |
| Key Colony Beach City | 797 | Florida | 26 |
| Gulf Stream Town | 786 | Florida | 27 |
| Webster City | 785 | Florida | 28 |
| White Springs Town | 777 | Florida | 29 |
| Gun Club Estates | 776 | Florida | 30 |
| Waverly | 767 | Florida | 31 |
| Fanning Springs City | 764 | Florida | 32 |
| Penney Farms Town | 749 | Florida | 33 |
| Solana | 742 | Florida | 34 |
| Lawtey City | 730 | Florida | 35 |
| Juno Ridge | 718 | Florida | 36 |
| Shalimar Town | 717 | Florida | 37 |
| Branford Town | 712 | Florida | 38 |
| Wiscon | 706 | Florida | 39 |
| Coleman City | 703 | Florida | 40 |
| Cedar Key City | 702 | Florida | 41 |
| Welaka Town | 701 | Florida | 42 |
| Pioneer | 697 | Florida | 43 |
| East Williston | 694 | Florida | 44 |
| Vernon City | 687 | Florida | 45 |
| Greenwood Town | 686 | Florida | 46 |
| Bradley Junction | 686 | Florida | 47 |
| Avalon | 679 | Florida | 48 |
| Matlacha | 677 | Florida | 49 |
| Garden Grove | 674 | Florida | 50 |
| Sea Ranch Lakes Village | 670 | Florida | 51 |
| Melbourne Village Town | 662 | Florida | 52 |
| Lemon Grove | 657 | Florida | 53 |
| Hosford | 650 | Florida | 54 |
| Paxton Town | 644 | Florida | 55 |
| Navarre Beach | 638 | Florida | 56 |
| Duck Key | 621 | Florida | 57 |
| Seville | 614 | Florida | 58 |
| Wabasso | 609 | Florida | 59 |
| Roeville | 608 | Florida | 60 |
| Greensboro Town | 602 | Florida | 61 |
| Briny Breezes Town | 601 | Florida | 62 |
| Micanopy Town | 600 | Florida | 63 |
| Ponce de Leon Town | 598 | Florida | 64 |
| St. Lucie Village Town | 590 | Florida | 65 |
| Captiva | 583 | Florida | 66 |
| Hastings Town | 580 | Florida | 67 |
| Fort White Town | 567 | Florida | 68 |
| Lake Hart | 542 | Florida | 69 |
| Laurel Hill City | 537 | Florida | 70 |
| Altha Town | 536 | Florida | 71 |
| Jay Town | 533 | Florida | 72 |
| Page Park | 514 | Florida | 73 |
| Tildenville | 511 | Florida | 74 |
| Reddick Town | 506 | Florida | 75 |
| Yankeetown Town | 502 | Florida | 76 |
| Lake Mystic | 500 | Florida | 77 |
| Hampton City | 500 | Florida | 78 |
| Alford Town | 489 | Florida | 79 |
| Ferndale | 472 | Florida | 80 |
| Brownsdale | 471 | Florida | 81 |
| Gardner | 463 | Florida | 82 |
| Spring Lake | 458 | Florida | 83 |
| Sopchoppy City | 457 | Florida | 84 |
| Bell Town | 456 | Florida | 85 |
| Mcintosh Town | 452 | Florida | 86 |
| Hillsboro Pines | 446 | Florida | 87 |
| Berrydale | 441 | Florida | 88 |
| Glen St. Mary Town | 437 | Florida | 89 |
| Acacia Villas | 427 | Florida | 90 |
| Trilby | 419 | Florida | 91 |
| Orchid Town | 415 | Florida | 92 |
| Caryville Town | 411 | Florida | 93 |
| Pineland | 407 | Florida | 94 |
| Manalapan Town | 406 | Florida | 95 |
| Jupiter Inlet Colony Town | 400 | Florida | 96 |
| Everglades City | 400 | Florida | 97 |
| Spring Ridge | 398 | Florida | 98 |
| Wacissa | 386 | Florida | 99 |
| Wausau Town | 383 | Florida | 100 |
| Cinco Bayou Town | 383 | Florida | 101 |
| Raleigh | 373 | Florida | 102 |
| Munson | 372 | Florida | 103 |
| Canal Point | 367 | Florida | 104 |
| Homeland | 366 | Florida | 105 |
| Esto Town | 364 | Florida | 106 |
| La Crosse Town | 360 | Florida | 107 |
| Chokoloskee | 359 | Florida | 108 |
| Ocean Breeze Park Town | 355 | Florida | 109 |
| Lee Town | 352 | Florida | 110 |
| Garcon Point | 347 | Florida | 111 |
| Beverly Beach Town | 338 | Florida | 112 |
| Brooker Town | 338 | Florida | 113 |
| Ona | 314 | Florida | 114 |
| Aripeka | 308 | Florida | 115 |
| Whitfield | 295 | Florida | 116 |
| St. Marks City | 293 | Florida | 117 |
| Westville Town | 289 | Florida | 118 |
| Nobleton | 282 | Florida | 119 |
| Waukeenah | 272 | Florida | 120 |
| Ebro Town | 270 | Florida | 121 |
| Goodland | 267 | Florida | 122 |
| Okahumpka | 267 | Florida | 123 |
| Lisbon | 260 | Florida | 124 |
| Mulat | 259 | Florida | 125 |
| Windsor | 256 | Florida | 126 |
| Raiford Town | 255 | Florida | 127 |
| Hillcrest Heights Town | 254 | Florida | 128 |
| Golf Village | 252 | Florida | 129 |
| Jacob City | 250 | Florida | 130 |
| Floridatown | 244 | Florida | 131 |
| Yeehaw Junction | 240 | Florida | 132 |
| Fort Green Springs | 231 | Florida | 133 |
| Highland Park Village | 230 | Florida | 134 |
| Campbellton Town | 230 | Florida | 135 |
| Matlacha Isles-matlacha Shores | 229 | Florida | 136 |
| Pine Level | 227 | Florida | 137 |
| Mount Carmel | 227 | Florida | 138 |
| Glen Ridge Town | 219 | Florida | 139 |
| Charleston Park | 218 | Florida | 140 |
| Lloyd | 215 | Florida | 141 |
| Noma Town | 211 | Florida | 142 |
| Layton City | 184 | Florida | 143 |
| Dixonville | 181 | Florida | 144 |
| Worthington Springs Town | 181 | Florida | 145 |
| Pittman | 180 | Florida | 146 |
| Lamont | 178 | Florida | 147 |
| Horseshoe Beach Town | 169 | Florida | 148 |
| Morriston | 164 | Florida | 149 |
| Plantation Island | 163 | Florida | 150 |
| Springhill | 160 | Florida | 151 |
| Fidelis | 156 | Florida | 152 |
| Sumatra | 148 | Florida | 153 |
| Dickerson City | 146 | Florida | 154 |
| Cloud Lake Town | 135 | Florida | 155 |
| Otter Creek Town | 134 | Florida | 156 |
| Limestone | 132 | Florida | 157 |
| Fisher Island | 132 | Florida | 158 |
| Bascom Town | 121 | Florida | 159 |
| Day | 116 | Florida | 160 |
| Istachatta | 116 | Florida | 161 |
| Belleair Shore Town | 109 | Florida | 162 |
| Fort Green | 101 | Florida | 163 |
| Aucilla | 100 | Florida | 164 |
| Altoona | 89 | Florida | 165 |
| Indian Creek Village | 86 | Florida | 166 |
| Lake Lindsey | 71 | Florida | 167 |
| Cobbtown | 67 | Florida | 168 |
| Pine Island | 64 | Florida | 169 |
| Bay Lake City | 47 | Florida | 170 |
| Lake Harbor | 45 | Florida | 171 |
| Bayport | 43 | Florida | 172 |
| Lazy Lake Village | 24 | Florida | 173 |
| Islandia City | 18 | Florida | 174 |
| Marineland Town | 16 | Florida | 175 |
| Weeki Wachee City | 12 | Florida | 176 |
| Lake Buena Vista City | 10 | Florida | 177 |
| Brewster | 3 | Florida | 178 |