| City Name | Population | State | Rank |
|---|
| Louisville/jefferson County Metro Government (balance) | 597,337 | Kentucky | 1 |
| Lexington-fayette Urban County | 295,803 | Kentucky | 2 |
| Bowling Green City | 58,067 | Kentucky | 3 |
| Owensboro City | 57,265 | Kentucky | 4 |
| Covington City | 40,640 | Kentucky | 5 |
| Hopkinsville City | 31,577 | Kentucky | 6 |
| Richmond City | 31,364 | Kentucky | 7 |
| Florence City | 29,951 | Kentucky | 8 |
| Georgetown City | 29,098 | Kentucky | 9 |
| Henderson City | 28,757 | Kentucky | 10 |
| Elizabethtown City | 28,531 | Kentucky | 11 |
| Nicholasville City | 28,015 | Kentucky | 12 |
| Jeffersontown City | 26,595 | Kentucky | 13 |
| Frankfort City | 25,527 | Kentucky | 14 |
| Paducah City | 25,024 | Kentucky | 15 |
| Independence City | 24,757 | Kentucky | 16 |
| Radcliff City | 21,688 | Kentucky | 17 |
| Ashland City | 21,684 | Kentucky | 18 |
| Madisonville City | 19,591 | Kentucky | 19 |
| Winchester City | 18,368 | Kentucky | 20 |
| Erlanger City | 18,082 | Kentucky | 21 |
| Murray City | 17,741 | Kentucky | 22 |
| St. Matthews City | 17,472 | Kentucky | 23 |
| Fort Thomas City | 16,325 | Kentucky | 24 |
| Danville City | 16,218 | Kentucky | 25 |
| Burlington | 15,926 | Kentucky | 26 |
| Newport City | 15,273 | Kentucky | 27 |
| Shively City | 15,264 | Kentucky | 28 |
| Shelbyville City | 14,045 | Kentucky | 29 |
| Glasgow City | 14,028 | Kentucky | 30 |
| Fort Campbell North | 13,685 | Kentucky | 31 |
| Berea City | 13,561 | Kentucky | 32 |
| Bardstown City | 11,700 | Kentucky | 33 |
| Shepherdsville City | 11,222 | Kentucky | 34 |
| Somerset City | 11,196 | Kentucky | 35 |
| Lyndon City | 11,002 | Kentucky | 36 |
| Lawrenceburg City | 10,505 | Kentucky | 37 |
| Middlesborough City | 10,334 | Kentucky | 38 |
| Fort Knox | 10,124 | Kentucky | 39 |
| Mayfield City | 10,024 | Kentucky | 40 |
| Mount Washington City | 9,117 | Kentucky | 41 |
| Campbellsville City | 9,108 | Kentucky | 42 |
| Oakbrook | 9,036 | Kentucky | 43 |
| Maysville City | 9,011 | Kentucky | 44 |
| Edgewood City | 8,575 | Kentucky | 45 |
| Versailles City | 8,568 | Kentucky | 46 |
| Paris City | 8,553 | Kentucky | 47 |
| Alexandria City | 8,477 | Kentucky | 48 |
| Elsmere City | 8,451 | Kentucky | 49 |
| Franklin City | 8,408 | Kentucky | 50 |
| Harrodsburg City | 8,340 | Kentucky | 51 |
| Fort Mitchell City | 8,207 | Kentucky | 52 |
| Hillview City | 8,172 | Kentucky | 53 |
| La Grange City | 8,082 | Kentucky | 54 |
| London City | 7,993 | Kentucky | 55 |
| Francisville | 7,944 | Kentucky | 56 |
| Oak Grove City | 7,489 | Kentucky | 57 |
| Villa Hills City | 7,489 | Kentucky | 58 |
| Flatwoods City | 7,423 | Kentucky | 59 |
| Corbin City | 7,304 | Kentucky | 60 |
| Middletown City | 7,218 | Kentucky | 61 |
| Russellville City | 6,960 | Kentucky | 62 |
| Highland Heights City | 6,923 | Kentucky | 63 |
| Pikeville City | 6,903 | Kentucky | 64 |
| Mount Sterling City | 6,895 | Kentucky | 65 |
| Morehead City | 6,845 | Kentucky | 66 |
| Leitchfield City | 6,699 | Kentucky | 67 |
| Taylor Mill City | 6,604 | Kentucky | 68 |
| Cynthiana City | 6,402 | Kentucky | 69 |
| Princeton City | 6,329 | Kentucky | 70 |
| Monticello City | 6,188 | Kentucky | 71 |
| Central City | 5,978 | Kentucky | 72 |
| Bellevue City | 5,955 | Kentucky | 73 |
| Hebron | 5,929 | Kentucky | 74 |
| Cold Spring City | 5,912 | Kentucky | 75 |
| Buckner | 5,837 | Kentucky | 76 |
| Fort Wright City | 5,723 | Kentucky | 77 |
| Lebanon City | 5,539 | Kentucky | 78 |
| Douglass Hills City | 5,484 | Kentucky | 79 |
| Union City | 5,379 | Kentucky | 80 |
| Dayton City | 5,338 | Kentucky | 81 |
| Williamsburg City | 5,245 | Kentucky | 82 |